An isothermal, label-free, and rapid one-step RNA amplification/detection assay for diagnosis of respiratory viral infections
Identifieur interne : 001380 ( Main/Exploration ); précédent : 001379; suivant : 001381An isothermal, label-free, and rapid one-step RNA amplification/detection assay for diagnosis of respiratory viral infections
Auteurs : Bonhan Koo [Corée du Sud] ; Choong Eun Jin [Corée du Sud] ; Tae Yoon Lee [Corée du Sud] ; Jeong Hoon Lee [Corée du Sud] ; Mi Kyoung Park [Singapour] ; Heungsup Sung [Corée du Sud] ; Se Yoon Park [Corée du Sud] ; Hyun Jung Lee [Corée du Sud] ; Sun Mi Kim [Corée du Sud] ; Ji Yeun Kim [Corée du Sud] ; Sung-Han Kim [Corée du Sud] ; Yong Shin [Corée du Sud]Source :
- Biosensors & Bioelectronics [ 0956-5663 ] ; 2016.
Descripteurs français
- KwdFr :
- ARN viral (génétique), ARN viral (isolement et purification), Coronavirus (génétique), Coronavirus (isolement et purification), Grippe humaine (diagnostic), Grippe humaine (génétique), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (génétique), Influenzavirus A (génétique), Influenzavirus A (isolement et purification), Influenzavirus B (génétique), Influenzavirus B (isolement et purification), Limite de détection, Maladies virales (diagnostic), Maladies virales (génétique), Techniques d'amplification d'acides nucléiques (), Techniques de biocapteur ().
- MESH :
- diagnostic : Grippe humaine, Infections à coronavirus, Maladies virales.
- génétique : ARN viral, Coronavirus, Grippe humaine, Infections à coronavirus, Influenzavirus A, Influenzavirus B, Maladies virales.
- isolement et purification : ARN viral, Coronavirus, Influenzavirus A, Influenzavirus B.
- Humains, Limite de détection, Techniques d'amplification d'acides nucléiques, Techniques de biocapteur.
English descriptors
- KwdEn :
- Biosensing Techniques (methods), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (genetics), Humans, Influenza, Human (diagnosis), Influenza, Human (genetics), Influenzavirus A (genetics), Influenzavirus A (isolation & purification), Influenzavirus B (genetics), Influenzavirus B (isolation & purification), Limit of Detection, Nucleic Acid Amplification Techniques (methods), RNA, Viral (genetics), RNA, Viral (isolation & purification), Virus Diseases (diagnosis), Virus Diseases (genetics).
- MESH :
- chemical , genetics : RNA, Viral.
- diagnosis : Coronavirus Infections, Influenza, Human, Virus Diseases.
- genetics : Coronavirus, Coronavirus Infections, Influenza, Human, Influenzavirus A, Influenzavirus B, Virus Diseases.
- isolation & purification : Coronavirus, Influenzavirus A, Influenzavirus B, RNA, Viral.
- methods : Biosensing Techniques, Nucleic Acid Amplification Techniques.
- Humans, Limit of Detection.
Abstract
Recently, RNA viral infections caused by respiratory viruses, such as influenza, parainfluenza, respiratory syncytial virus, coronavirus, and Middle East respiratory syndrome-coronavirus (MERS-CoV), and Zika virus, are a major public health threats in the world. Although myriads of diagnostic methods based on RNA amplification have been developed in the last decades, they continue to lack speed, sensitivity, and specificity for clinical use. A rapid and accurate diagnostic method is needed for appropriate control, including isolation and treatment of the patients. Here, we report an isothermal, label-free, one-step RNA amplification and detection system, termed as iROAD, for the diagnosis of respiratory diseases. It couples a one-step isothermal RNA amplification method and a bio-optical sensor for simultaneous viral RNA amplification/detection in a label-free and real-time manner. The iROAD assay offers a one-step viral RNA amplification/detection example to rapid analysis (<20 min). The detection limit of iROAD assay was found to be 10-times more sensitive than that of real-time reverse transcription-PCR method. We confirmed the clinical utility of the iROAD assay by detecting viral RNAs obtained from 63 human respiratory samples. We envision that the iROAD assay will be useful and potentially adaptable for better diagnosis of emerging infectious diseases including respiratory diseases.
Url:
DOI: 10.1016/j.bios.2016.11.051
PubMed: 27894035
PubMed Central: 7127409
Affiliations:
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isothermal, label-free, and rapid one-step RNA amplification/detection assay for diagnosis of respiratory viral infections</title><author><name sortKey="Koo, Bonhan" sort="Koo, Bonhan" uniqKey="Koo B" first="Bonhan" last="Koo">Bonhan Koo</name><affiliation wicri:level="3"><nlm:aff id="aff0005">Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Biomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Biomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Jin, Choong Eun" sort="Jin, Choong Eun" uniqKey="Jin C" first="Choong Eun" last="Jin">Choong Eun Jin</name><affiliation wicri:level="3"><nlm:aff id="aff0005">Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Biomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Biomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Lee, Tae Yoon" sort="Lee, Tae Yoon" uniqKey="Lee T" first="Tae Yoon" last="Lee">Tae Yoon Lee</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Technology Education, Chungnam National University, Daejeon 34134, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Technology Education, Chungnam National University, Daejeon 34134</wicri:regionArea><wicri:noRegion>Daejeon 34134</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Jeong Hoon" sort="Lee, Jeong Hoon" uniqKey="Lee J" first="Jeong Hoon" last="Lee">Jeong Hoon Lee</name><affiliation wicri:level="1"><nlm:aff id="aff0015">Department of Electrical Engineering, Kwangwoon University, 447-1 Wolgye, Nowon, Seoul 136-791, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Electrical Engineering, Kwangwoon University, 447-1 Wolgye, Nowon, Seoul 136-791</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Park, Mi Kyoung" sort="Park, Mi Kyoung" uniqKey="Park M" first="Mi Kyoung" last="Park">Mi Kyoung Park</name><affiliation wicri:level="1"><nlm:aff id="aff0020">One BioMed Pte Ltd, 60 Biopolis street, Genome #02-01, Singapore 138672, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>One BioMed Pte Ltd, 60 Biopolis street, Genome #02-01, Singapore 138672</wicri:regionArea><wicri:noRegion>Singapore 138672</wicri:noRegion></affiliation></author><author><name sortKey="Sung, Heungsup" sort="Sung, Heungsup" uniqKey="Sung H" first="Heungsup" last="Sung">Heungsup Sung</name><affiliation wicri:level="3"><nlm:aff id="aff0025">Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Laboratory Medicine, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Park, Se Yoon" sort="Park, Se Yoon" uniqKey="Park S" first="Se Yoon" last="Park">Se Yoon Park</name><affiliation wicri:level="3"><nlm:aff id="aff0030">Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff0035">Division of Infectious Diseases, Department of Internal Medicine, Soonchunhyang University College of Medicine, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Infectious Diseases, Department of Internal Medicine, Soonchunhyang University College of Medicine, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Lee, Hyun Jung" sort="Lee, Hyun Jung" uniqKey="Lee H" first="Hyun Jung" last="Lee">Hyun Jung Lee</name><affiliation wicri:level="3"><nlm:aff id="aff0030">Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Sun Mi" sort="Kim, Sun Mi" uniqKey="Kim S" first="Sun Mi" last="Kim">Sun Mi Kim</name><affiliation wicri:level="3"><nlm:aff id="aff0030">Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Ji Yeun" sort="Kim, Ji Yeun" uniqKey="Kim J" first="Ji Yeun" last="Kim">Ji Yeun Kim</name><affiliation wicri:level="3"><nlm:aff id="aff0030">Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Sung Han" sort="Kim, Sung Han" uniqKey="Kim S" first="Sung-Han" last="Kim">Sung-Han Kim</name><affiliation wicri:level="3"><nlm:aff id="aff0030">Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Infectious Diseases, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympicro-43gil, Songpa-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Shin, Yong" sort="Shin, Yong" uniqKey="Shin Y" first="Yong" last="Shin">Yong Shin</name><affiliation wicri:level="3"><nlm:aff id="aff0005">Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Biomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, Seoul, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Biomedical Engineering Research Center, Asan Institute of Life Sciences, Asan Medical Center, 88 Olympicro-43gil, Songpa-gu, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Biosensors & Bioelectronics</title><idno type="ISSN">0956-5663</idno><idno type="eISSN">1873-4235</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (methods)</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (genetics)</term><term>Humans</term><term>Influenza, Human (diagnosis)</term><term>Influenza, Human (genetics)</term><term>Influenzavirus A (genetics)</term><term>Influenzavirus A (isolation & purification)</term><term>Influenzavirus B (genetics)</term><term>Influenzavirus B (isolation & purification)</term><term>Limit of Detection</term><term>Nucleic Acid Amplification Techniques (methods)</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (isolation & purification)</term><term>Virus Diseases (diagnosis)</term><term>Virus Diseases (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>ARN viral (isolement et purification)</term><term>Coronavirus (génétique)</term><term>Coronavirus (isolement et purification)</term><term>Grippe humaine (diagnostic)</term><term>Grippe humaine (génétique)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (génétique)</term><term>Influenzavirus A (génétique)</term><term>Influenzavirus A (isolement et purification)</term><term>Influenzavirus B (génétique)</term><term>Influenzavirus B (isolement et purification)</term><term>Limite de détection</term><term>Maladies virales (diagnostic)</term><term>Maladies virales (génétique)</term><term>Techniques d'amplification d'acides nucléiques ()</term><term>Techniques de biocapteur ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Influenza, Human</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term><term>Infections à coronavirus</term><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term><term>Coronavirus Infections</term><term>Influenza, Human</term><term>Influenzavirus A</term><term>Influenzavirus B</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Coronavirus</term><term>Grippe humaine</term><term>Infections à coronavirus</term><term>Influenzavirus A</term><term>Influenzavirus B</term><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term><term>Influenzavirus A</term><term>Influenzavirus B</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term><term>Coronavirus</term><term>Influenzavirus A</term><term>Influenzavirus B</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>Nucleic Acid Amplification Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Limit of Detection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Limite de détection</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Recently, RNA viral infections caused by respiratory viruses, such as influenza, parainfluenza, respiratory syncytial virus, coronavirus, and Middle East respiratory syndrome-coronavirus (MERS-CoV), and Zika virus, are a major public health threats in the world. Although myriads of diagnostic methods based on RNA amplification have been developed in the last decades, they continue to lack speed, sensitivity, and specificity for clinical use. A rapid and accurate diagnostic method is needed for appropriate control, including isolation and treatment of the patients. Here, we report an isothermal, label-free, one-step RNA amplification and detection system, termed as iROAD, for the diagnosis of respiratory diseases. It couples a one-step isothermal RNA amplification method and a bio-optical sensor for simultaneous viral RNA amplification/detection in a label-free and real-time manner. The iROAD assay offers a one-step viral RNA amplification/detection example to rapid analysis (<20 min). The detection limit of iROAD assay was found to be 10-times more sensitive than that of real-time reverse transcription-PCR method. We confirmed the clinical utility of the iROAD assay by detecting viral RNAs obtained from 63 human respiratory samples. We envision that the iROAD assay will be useful and potentially adaptable for better diagnosis of emerging infectious diseases including respiratory diseases.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Agrawal, N" uniqKey="Agrawal N">N. Agrawal</name></author><author><name sortKey="Hassan, Y A" uniqKey="Hassan Y">Y.A. Hassan</name></author><author><name sortKey="Ugaz, V M" uniqKey="Ugaz V">V.M. Ugaz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Amer, H M" uniqKey="Amer H">H.M. Amer</name></author><author><name sortKey="El Wahed, A A" uniqKey="El Wahed A">A.A. El Wahed</name></author><author><name sortKey="Shalaby, M A" uniqKey="Shalaby M">M.A. Shalaby</name></author><author><name sortKey="Almajhdi, F N" uniqKey="Almajhdi F">F.N. Almajhdi</name></author><author><name sortKey="Hufert, F T" uniqKey="Hufert F">F.T. 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Chen</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Corée du Sud</li><li>Singapour</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Koo, Bonhan" sort="Koo, Bonhan" uniqKey="Koo B" first="Bonhan" last="Koo">Bonhan Koo</name></region><name sortKey="Jin, Choong Eun" sort="Jin, Choong Eun" uniqKey="Jin C" first="Choong Eun" last="Jin">Choong Eun Jin</name><name sortKey="Kim, Ji Yeun" sort="Kim, Ji Yeun" uniqKey="Kim J" first="Ji Yeun" last="Kim">Ji Yeun Kim</name><name sortKey="Kim, Sun Mi" sort="Kim, Sun Mi" uniqKey="Kim S" first="Sun Mi" last="Kim">Sun Mi Kim</name><name sortKey="Kim, Sung Han" sort="Kim, Sung Han" uniqKey="Kim S" first="Sung-Han" last="Kim">Sung-Han Kim</name><name sortKey="Lee, Hyun Jung" sort="Lee, Hyun Jung" uniqKey="Lee H" first="Hyun Jung" last="Lee">Hyun Jung Lee</name><name sortKey="Lee, Jeong Hoon" sort="Lee, Jeong Hoon" uniqKey="Lee J" first="Jeong Hoon" last="Lee">Jeong Hoon Lee</name><name sortKey="Lee, Tae Yoon" sort="Lee, Tae Yoon" uniqKey="Lee T" first="Tae Yoon" last="Lee">Tae Yoon Lee</name><name sortKey="Park, Se Yoon" sort="Park, Se Yoon" uniqKey="Park S" first="Se Yoon" last="Park">Se Yoon Park</name><name sortKey="Park, Se Yoon" sort="Park, Se Yoon" uniqKey="Park S" first="Se Yoon" last="Park">Se Yoon Park</name><name sortKey="Shin, Yong" sort="Shin, Yong" uniqKey="Shin Y" first="Yong" last="Shin">Yong Shin</name><name sortKey="Sung, Heungsup" sort="Sung, Heungsup" uniqKey="Sung H" first="Heungsup" last="Sung">Heungsup Sung</name></country><country name="Singapour"><noRegion><name sortKey="Park, Mi Kyoung" sort="Park, Mi Kyoung" uniqKey="Park M" first="Mi Kyoung" last="Park">Mi Kyoung Park</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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